Even though agronomists have considered the spatial root distribution of plants to be important for interspecific interactions in agricultural intercropping, few experimental studies have quantified patterns of root distribution and their impacts on interspecific interactions in agroforestry systems. A field experiment was conducted to investigate the relationship between root distribution and interspecific interactions between intercropped jujube tree (Zizyphus jujuba Mill.) and wheat (Triticum aestivum Linn.) in Hetian, south Xinjiang province, northwest China. Roots were sampled by auger in 2‐, 4‐ and 6‐year‐old jujube tree/wheat intercropping and in sole wheat and 2‐, 4‐ and 6‐year‐old sole jujube down to 100 cm depth in the soil profile. The roots of both intercropped wheat and jujube had less root length density (RLD) at all soil depths than those of sole wheat and jujube trees. The RLD of 6‐year‐old jujube intercropped with wheat at different soil depths was influenced by intercropping to a smaller extent than in other jujube/wheat intercropping combinations. 6‐year‐old jujube exhibited a stronger negative effect on the productivity of wheat than did 2‐ or 4‐year‐old jujube and there was less effect on productivity of jujube in the 6‐year‐old system than in the 2‐ or 4‐year‐old jujube trees grown in monoculture. These findings may partly explain the interspecific competition effects in jujube tree/wheat agroforestry systems.