In this paper, the on-line k-truck transportation problem (k-OLTTP) whose objects are to be transported between the vertices of a given graph on which there are k mobile trucks to be scheduled is proposed. It is motivated by the research concerning on-line k-truck problem and on-line transportation problem. The goal is to minimize the makespan which is consumed to complete some on-line request sequence. Some preliminary knowledge is introduced and the model of k-OLTTP is established firstly. Two versions of a special case of k-OLTTP, namely 1-OLTTP, have been studied and some results are obtained. For the first version, Open-1-OLTTP, a lower bound of competitive ratio 2 is presented and two optimal on-line algorithms, Reschedule Strategy (RS) and Lay Over Strategy (LOS) respectively, are analyzed. For the second version, Close-1-OLTTP, a lower bound of competitive ratio ½+½·√(1+4/θ) , where θ is the ratio between the time consumed by the loaded truck and the empty truck to travel the same distance, is also developed and on-line algorithms RS and LOS are proved to have competitive ratio 2. Finally, some interesting remarks concerning OLTTP and its future research are discussed.